臍帶來源間充質(zhì)干細(xì)胞抑制巨噬細(xì)胞M1極化
中國(guó)免疫學(xué)雜志
頁數(shù): 7 2024-04-19
摘要: 目的:探究人臍帶間充質(zhì)干細(xì)胞(hUC-MSCs)對(duì)巨噬細(xì)胞M1/M2極化的作用。方法:將hUC-MSCs與佛波酯(PMA)誘導(dǎo)分化的巨噬細(xì)胞樣細(xì)胞(pTHP-1巨噬細(xì)胞)共培養(yǎng)后進(jìn)行轉(zhuǎn)錄組測(cè)序分析,篩選差異表達(dá)基因,進(jìn)一步進(jìn)行GO及KEGG富集分析。細(xì)胞增殖實(shí)驗(yàn)(CCK-8和EdU)分析hUC-MSCs對(duì)pTHP-1細(xì)胞增殖的影響。流式細(xì)胞術(shù)檢測(cè)hUC-MSCs對(duì)LPS刺激的p... (共7頁)