Addition of tanshinone IIA to UW solution decreases skeletal muscle ischemia-reperfusion injury

摘要： <正> Aim:To investigate whether tanshinone ⅡA could improve the effect of UWsolution for skeletal muscle preservation and to determine the dose range oftanshinone ⅡA providing optimal protection during ischemia and reperfusion.Methods:Ischemic rat limbs were perfused with UW solution or UW plus tanshinoneⅡA (UW+T,0.05,0.1,or 0.2 mg/mL) for 0.5h before reperfusion;controls (I/R)received no perfusion.Serum creatine phosphokinase (CPK),aspartate aminotrans-ferase (AST),and lactate dehydrogenase (LDH) were measured pre-ischemia andafter reperfusion (2-h,4-h,and 6-h).Muscle water content,superoxide dismutase(SOD),malondialdehyde (MDA),adenosine triphosphatase (ATPase) were as-sessed pre-reperfusion and after 6-h reperfusion.Intercellular adhesion molecule-1(ICAM-1) and apoptosis were detected after 6-h reperfusion.Reperfusion bloodflow was monitored during reperfusion period.Results:UW and UW+T pre-vented luxury perfusion during reperfusion and inhibited ICAM-1 expression andapoptosis after 6-h reperfusion.Serum CPK,AST,and LDH levels in UW ratswere significantly less than those in controls after 2-h reperfusion (no difference,4-h or 6-h reperfusion).After 4-h ischemia,there were significant differences inwater content,MDA,SOD,and ATPase between UW and controls,but no differ-ence after 6-h reperfusion.All tests with UW+T rats were significantly differentfrom control results at corresponding durations.Higher tanshinone doses im-proved results.Conclusion:UW plus tanshinone ⅡA increased protection againstI/R injury,suggesting that tanshinone ⅡA has clinical value. （共9頁(yè)）