Velvetantlerpolypeptideisabletoinducedifferentiationofneuralstemcellstowardsneuronsinvitro
JournalofTraditionalChineseMedicine
頁數(shù)： 6 2017-06-15
摘要： OBJECTIVE:Toinvestigatetheneuraldifferentiationcapacityofwaterextractionofvelvetantler.METHODS:Velvetantler(CervusNipponTemminck)polypeptide(VAP)wasusedtodifferentiateneuralstemcells(NSCs)towardsneuronsinthestudy.Firstly,weobtainthepolypeptidesofVAPbywaterextraction.Secondly,weobservedthemorphology,assayedthefactorsinthemediabyenzyme-linkedimmunosorbentassay,anddetectedthespecialneuralmoleculesbyimmunofluorescencestaining.NSCswereculturedonthecellclimbingfilm.Afterneuronaldifferentiation,differentiatedNSCsweremountedforimmunocytochemistrywithimmunofluorescencetechnique.RESULTS:Thedifferentiatingcellslooklikeneuron,somespecialfactors,suchasGlialcellline-derivedneurotrophicfactor,nervegrowthfactor,inthemediacanbedetectedwhiledifferentiatedneuron-likecellscanexpressthespecialneuralmolecules.CONCLUSION:DifferentiationofNSCstowardsneuronscanbeinducedbyvelvetantlerpolypeptide. （共6頁）
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